Eficacia antibacteriana de tres selladores endodónticos frente al Enterococcus faecalis / Antibacterial effectiveness of three endodontic sealers against Enterococcus faecalis

Objetivos: Este estudio tiene como objetivo la comparación de la eficacia antibacteriana sobre el Enterococcus faecalis, de tres cementos selladores de obturación utilizados en la terapia endodóntica, determinada mediante la medición del tamaño del halo inhibitorio. Material y métodos: se realizó un estudio comparativo in vitro, en el cual se utilizó el método de difusión en el agar de Müller- Hinton. Se calculó una muestra de 10 replicaciones por cemento haciendo un total de 30 unidades de estudio (mediante el programa G Power 3.1). Los datos del halo inhibitorio fueron medidos en milímetros después de 24 horas de incubación, a una temperatura de 37°C, los resultados obtenidos se anotaron en una ficha elaborada por el autor, para posteriormente procesarlos mediante el análisis estadístico de Shapiro-Wilk, Kruskal-Wallis y U de Mann Whitney. Resultados: El sellador a base de resina epóxica (TOPSEAL) presentó un halo de inhibición de 7,7 mm, el sellador a base de hidróxido de calcio (SEALAPEX) provocó un halo inhibitorio de 6,0 mm y el sellador a base de óxido de zinc y eugenol (GROSSFAR) presentó un halo de inhibición de 8,4 mm, siendo el de mayor eficacia de los tres selladores utilizados en este estudio. Conclusiones: Con este estudio se llegó a la conclusión que los selladores estudiados no son iguales entre si con respecto a su eficacia antibacteriana contra el Enterococcus faecalis.

Objectives: The aim of this study was the comparison of antibacterial effectiveness of three endodontic sealers, used in endodontic therapy. The antibacterial effectiveness of sealers was determinate by measuring the inhibitory halo against Enterococcus faecalis. Material and methods: A comparative in vitro study was done; the method of agar diffusion of Müller- Hinton was used. A sample of 10 replications for each sealer was estimated with the help the program (G Power 3.1) the total of sample was the 30 units of study. The data of inhibitory halo was measured in millimeters after 24 hours, incubated a temperature of 37°C, the results was registered in a format developed by the author, later the results were processed by statistical analysis Shapiro-Wilk, Kruskal-Wallis and U de Mann Whitney. Results: The resin- based sealer (TOPSEAL) showed an inhibitory halo of 7.7 mm; the hydroxide of calcium - based sealer (SEALAPEX) was of 6.0 mm whereas the oxide of zinc and eugenol - based sealer showed an inhibitory halo of 8.4 mm, this inhibitory halo was the biggest in this study. Conclusions: This study concluded that the sealers used in this study are not equal in their antibacterial effectiveness against Enterococcus faecalis.

The effect of glass ionomer and adhesive cements on substance P expression in human dental pulp

OBJECTIVES: The purpose of this study was to quantify the effect of glass ionomer and adhesive cements on SP expression in healthy human dental pulp. Study DESIGN: Forty pulp samples were obtained from healthy premolars where extraction was indicated for orthodontic reasons. In thirty of these premolars a Class V cavity preparation was performed and teeth were equally divided in three groups: Experimental Group I: Glass Ionomer cement was placed in the cavity. Experimental Group II: Adhesive Cement was placed in the cavity. Positive control group: Class V cavities only. The remaining ten healthy premolars where extracted without treatment and served as a negative control group. All pulp samples were processed and SP was measured by radioimmunoassay. RESULTS: Greater SP expression was found in the adhesive cement group, followed by the glass ionomer and the positive control groups. The lower SP values were for the negative control group. ANOVA showed statistically significant differences between groups (p < 0.0001). Tukey HSD post hoc tests showed statistically significant differences in SP expression between negative control group and the 3 other groups (p < 0.01). Differences between the cavity-only group and the two experimental groups were also statistically significant (p < 0.05 and p < 0.01 respectively). There is also a statistically significant difference between the two experimental groups (p < 0.01). CONCLUSIONS: These findings suggest that adhesive cements provoke a greater SP expression when compared with glass ionomer

The effect of glass ionomer and adhesive cements on substance P expression in human dental pulp.

OBJECTIVES: The purpose of this study was to quantify the effect of glass ionomer and adhesive cements on SP expression in healthy human dental pulp. STUDY DESIGN: Forty pulp samples were obtained from healthy premolars where extraction was indicated for orthodontic reasons. In thirty of these premolars a Class V cavity preparation was performed and teeth were equally divided in three groups: Experimental Group I: Glass Ionomer cement was placed in the cavity. Experimental Group II: Adhesive Cement was placed in the cavity. Positive control group: Class V cavities only. The remaining ten healthy premolars where extracted without treatment and served as a negative control group. All pulp samples were processed and SP was measured by radioimmunoassay. RESULTS: Greater SP expression was found in the adhesive cement group, followed by the glass ionomer and the positive control groups. The lower SP values were for the negative control group. ANOVA showed statistically significant differences between groups (p<0.0001). Tukey HSD post hoc tests showed statistically significant differences in SP expression between negative control group and the 3 other groups (p<0.01). Differences between the cavity-only group and the two experimental groups were also statistically significant (p<0.05 and p<0.01 respectively). There is also a statistically significant difference between the two experimental groups (p<0.01). CONCLUSION: These findings suggest that adhesive cements provoke a greater SP expression when compared with glass ionomer.